Table 1.
Differentially expressed sporulation related proteins in kinA-overexpressed mutant spores of B. subtilis.
| Protein | Log2(M+/M−) | Log2(M+/WT+) | Function | Product |
|---|---|---|---|---|
| Upregulation | ||||
| CotQ | 1.66 | 1.70 | protection of the spore | spore coat protein |
| CotU | 2.49 | 2.00 | resistance of the spore | outer spore coat protein |
| GerE | 2.70 | 2.90 | regulation of SigK-dependent gene expression | transcriptional regulator (LuxR-FixJ family) |
| YheC | 3.07 | 3.17 | Unknown | ATP-binding spore coat protein |
| YhxC | 2.03 | 1.24 | Unknown | unknown |
| YjqC | 1.66 | 2.49 | protection of the spore | spore coat protein |
| YpqA | 1.31 | 2.14 | Unknown | unknown |
| YraF | 1.41 | 1.09 | Unknown | unknown |
| YtcC | 2.05 | 2.46 | lipopolysaccharide biosynthesis | sporulation protein |
| YxeD | 1.29 | 1.47 | Unknown | unknown |
| CotC | 1.51 | N.A. | resistance of the spore | spore coat protein (outer) |
| CotJA | 1.31 | N.A. | polypeptide composition of the spore coat | unknown |
| CotJB | 1.30 | N.A. | polypeptide composition of the spore coat | unknown |
| CotJC | 1.32 | N.A. | polypeptide composition of the spore coat, may protect against oxidative stress | putative manganese catalase |
| GerT | 1.86 | N.A. | germination | spore coat protein |
| YdhD | 1.31 | N.A. | Unknown | spore coat glycosylase |
| YqfT | 1.03 | N.A. | Unknown | unknown |
| YraG | 1.33 | N.A. | Unknown | unknown |
| KinA | 4.11 a | 5.29 | initiation of sporulation | two-component sensor kinase |
| MurG | N.A. | 1.13 | peptidoglycan precursor biosynthesis | UDP-N-acetylglucosamine-N-acetylmuramyl-(pentapeptide)pyrophosphoryl-undecaprenol N-acetylglucosamine transferase |
| SpsB | N.A. | 1.71 | spore coat polysaccharide synthesis | unknown |
| YabG | N.A. | 1.03 | modification of spore coat proteins | protease |
| YojB | N.A. | 1.26 | Unknown | unknown |
| YraD | N.A. | 1.15 | Unknown | unknown |
| Downregulation | ||||
| OppA | −4.16 | −2.53 | initiation of sporulation, competence development | oligopeptide ABC transporter (binding protein) |
| OppD | −2.93 | −1.42 | initiation of sporulation, competence development | oligopeptide ABC transporter (ATP-binding protein) |
| OppF | −3.04 | −1.84 | initiation of sporulation, competence development | oligopeptide ABC transporter (ATP-binding protein) |
| PdaA | −1.01 | −1.12 | spore cortex peptidoglycan synthesis | N-acetylmuramic acid deacetylase |
| SspA | −1.18 | −1.59 | protection of spore DNA | small acid-soluble spore protein (major alpha-type SASP) |
| YckD | −1.14 | −1.43 | Unknown | unknown |
| YugP | −1.02 | −1.20 | Unknown | unknown |
| CgeA | −3.27 | N.A. | maturation of the outermost layer of the spore | spore crust protein |
| GlnH | −2.49 | N.A. | glutamine uptake | glutamine ABC transporter (binding protein) |
| ParA | −1.01 | N.A. | forespore chromosome partitioning/negative regulation of Sporulation initiation | negative regulator of Sporulation initiation |
| PbpF | −1.10 | N.A. | bifunctional glucosyltransferase/transpeptidase | penicillin-binding protein 2C |
| YbfJ | −1.14 | N.A. | Unknown | unknown |
| YhfN | −1.02 | N.A. | Unknown | unknown |
| YuaG | −2.22 | N.A. | involved in the control of membrane fluidity | membrane-associated scaffold protein |
| BdbD | N.A. | −1.40 | oxidative folding of proteins | thiol-disulfide oxidoreductase |
| CotG | N.A. | −1.10 | resistance of the spore | spore coat protein |
| CotW | N.A. | −1.34 | resistance of the spore | spore crust protein (insoluble fraction) |
| CotX | N.A. | −1.31 | spore crust assembly | spore crust protein (insoluble fraction) |
| DacB | N.A. | −1.06 | carboxypeptidase | penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase |
| GerBC | N.A. | −1.28 | germination | nutrient receptor |
| OppC | N.A. | −1.52 | initiation of sporulation, competence development | oligopeptide ABC transporter (permease) |
| PhoP | N.A. | −1.55 | regulation of phosphate metabolism (phoA, phoB, phoD, resABCDE, tagA-tagB, tagDEF, [tuaA-H]) | two-component response regulator (OmpR family) |
| PhoR | N.A. | −1.93 | regulation of phosphate metabolism | two-component sensor kinase |
| spoVD | N.A. | −1.30 | spore morphogenesis | penicillin-binding protein (spore cortex) |
| SspE | N.A. | −1.09 | Unknown | small acid-soluble spore protein (major gamma-type SASP) |
| SspG | N.A. | −1.01 | protection of spore DNA | small acid-soluble spore protein (minor) |
| YbbC | N.A. | −1.32 | Unknown | unknown |
| YdcC | N.A. | −1.30 | Unknown | unknown |
| YjaZ | N.A. | −1.32 | Unknown | unknown |
a, is quantified in one replica. N.A.= not applicable; proteins were identified but not quantified.