Figure 4.

Flow cytometry assays. (A) MCF-cells treated with the peptide ²⁶[F] (15 µM) in the presence of PI and SYTO9 fluorophores. Negative control: cells without treatment, vehicle (DMEM); positive control: cells treated with Actinomycin-D for 24 h. (B) MCF-7 cells treated with the peptide ²⁶[F] in the presence of Annexin V and PI fluorophores for 24 h. Negative control: untreated cells, vehicle (DMEM); necrosis control: cells treated with 5 mM EDTA for 60 min; apoptosis control: cells treated with 15 µM Actinomycin-D for 24 h. (C) MCF-7 cells treated with the peptide ²⁶[F] in the presence of JC-1 fluorophore. Negative control: untreated cells, vehicle (DMEM); positive control: cells treated with 15 µM Actinomycin-D for 24 h.