Figure 3.

Metabolic profiling revealed tricarboxylic acid cycle (TCA-cycle) intermediates and anaplerotic amino acids were increased in early recurrent PDAC. (a) Partial least square discriminant analysis of PDOs derived from early (red) and late (green) recurrent PDAC patient unveiled global metabolic alterations. (b) Global pathway analysis revealed different pathways affected in PDOs. Identity of the pathways matching the cut-off values (−log(p) > 4 and pathway impact > 0.2) are depicted in table 2. (c) Volcano plot analysis of identified metabolites. Early recurrent to late recurrent fold changes are displayed as log2 values. Only top five altered metabolites are labeled. Each data point represents at least three technical replicates of the different PDOs.