Figure 1.

Neutrophil extracellular traps (NETs) alter cell morphology and enhance MCF7 migration in vitro. (a) Representative images of MCF7 cells that were cultured for 16 h in the absence (left) or the presence (right) of NETs (500 ng/mL). Magnification 100× and 400×, scale bar 500 µm and 100 µm, respectively. (b) Tumor cell migration was evaluated employing the Boyden chamber assay. MCF7 cells that were cultured for 16 h in the absence or the presence of NETs (500 ng/mL) were seeded in the upper chamber (5 × 10⁴ cells/well) and further allowed to migrate for 20 h. As chemoattractant, medium supplemented with fetal bovine serum (FBS) (2% or 10%) was used in lower chambers. Representative images of the migration assay are shown on the left panel (200× magnification). Migrated cells were quantified, and results are shown on the right panel. Data are presented as mean ± SD from three independent experiments. Statistical analysis of each condition was evaluated by unpaired t-test. Significance was assumed for * p < 0.05, *** p < 0.001.