Figure 4.
Pro-tumoral and pro-inflammatory mediators are regulated by NETs. MCF7 cells (5 × 105) were starved and further cultured for 16 h in the absence (grey bar) or the presence (black bar) of NETs (500 ng/mL). Genes analyzed: interleukin-1β (IL-1β/IL1B), interleukin-6 (IL-6/IL6), interleukin-8 (IL-8/CXCL8), CXCR1, matrix metalloprotease-2 (MMP-2/MMP2), and MMP9. MDA-MB-231 cells (5 × 105) were cultured for 3 h in the absence or the presence of NETs (500 ng/mL). Genes analyzed: IL1B, CXCL8, and cyclooxygenase-2 (COX-2/PTGS2). Gene expression was evaluated by quantitative RT-PCR using the ΔΔCT method. GAPDH was used as the reference gene. Columns represent means ± SD of three independent experiments. Statistical analysis was performed using unpaired t-test. * p < 0.05 and ** p < 0.01.