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. 2020 Jun 9;12(6):1503. doi: 10.3390/cancers12061503

Figure 4.

Figure 4

Changes in protein levels in OVCAR8-derived olaparib-resistant clones. Protein lysates were made from the OVCAR8 parent grown in normal medium and OVCAR8 clones maintained continuously under olaparib selection. (A) Western blots for 53BP1, BRCA1, RIF1, PARP1, PARG, MDR1, MUS81, REV7, EZH2, H3K27Me3, γH2AX from OVCAR8 and four olaparib-resistant OVCAR8 derived clones, with GAPDH as loading control. (B) Histograms showing levels of the different proteins in each clone, corrected for differences in loading control, and expressed relative to the level in the OVCAR8 parent. OVCAR8 parent shown in blue. OVCAR8 clones OVCAR8 OLA_12:1, OVCAR8 OLA_12:3, OVCAR8 OLA_12:4, and OVCAR8 OLA_12:5, resistant to 12.5 µM olaparib, are shown in dark pink. Information supporting the identification of proteins in these Western blots is shown in Figure S1.