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. 2020 Jun 22;21(12):4442. doi: 10.3390/ijms21124442

Figure 1.

Figure 1

Multipotent stromal cells (MSC) response to adenylate cyclase and protein kinase A (PKA) activation. (a) Immunofluorescent staining of the catalytic subunit of protein kinase A in MSC. Scale bar 100 µm. (b) Share of the cells responding to adenylate cyclase activator forskolin (10−6 M) and cell-permeable direct activator of protein kinase A 6-Bnz-cyclic AMP (10−4 M). Results are presented as mean ± SE, n = 17–24 in 4 independent experiments on the material of 3 different donors. (c) Representative field of view of the cells responding to the sequential adding of forskolin and 6-Bnz-cAMP. Yellow arrow marks the cell responded to the forskolin added. Blue arrows show the cells responded to 6-Bnz-cAMP added. Scale bar 100 µm. (d) Expression of mRNA of different isoforms of adenylyl cyclase in human MSC population. Raw data of scRNAseq analysis of MSC were downloaded from Reference [19].