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. 2020 Jun 25;21(12):4532. doi: 10.3390/ijms21124532

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of LDIR exposure on the expressions of M1/M2 markers in BV-2-stimulated cells. BV-2 cells were treated with LPS at a dose of 20 ng/mL for 24 h then exposed to LDIR (1 Gy/ 1 fraction). Cells were harvested at 12 and 24 h after irradiation. (A,B) The mRNA levels of M1 microglial marker CD86 and M2 microglial marker CD206 were measured by qRT-PCR. a indicated that comparison of control and LPS+LDIR 12 h (p < 0.05), b indicated that comparison of control and LPS+LDIR 24 h (p < 0.001), c indicated that comparison of LPS and LPS+LDIR 12 h (p < 0.05), d indicated that comparison of LPS and LPS+LDIR 24 h (p < 0.001), and e indicated that comparison of LDIR and LPS+LDIR 24 h (p < 0.05). Data are presented as mean ± SD from triplicate experiments.