TABLE 2:
Summary of results from the confocal microscopy screen for a subset of 21 randomly selected genes.
| Confocal screen results | |||||
|---|---|---|---|---|---|
| Gene name | Number of nuclei | Mean EFC ratio | Standard error | Statistically significant? | Hit in the high-throughput screen? |
| MTA2 | 176 | 4.62 | 0.15 | Yes | Yes |
| TAF7 | 255 | 5.07 | 0.10 | Yes | Yes |
| FOXP1 | 153 | 5.19 | 0.16 | Yes | Yes |
| DIDO1 | 146 | 5.14 | 0.15 | Yes | Yes |
| SUZ12 | 164 | 5.19 | 0.15 | Yes | Yes |
| TP53 | 235 | 5.35 | 0.13 | Yes | Yes |
| ARID4A | 176 | 6.01 | 0.20 | Yes | Yes |
| RAD54L2 | 165 | 5.49 | 0.13 | Yes | Yes |
| CXXC1 | 199 | 5.93 | 0.17 | Yes | Yes |
| MAP3K7 | 137 | 5.97 | 0.15 | No | Yes |
| FBL | 246 | 6.26 | 0.13 | No | No |
| RAD51 | 188 | 6.33 | 0.16 | No | No |
| WSB2 | 492 | 6.38 | 0.11 | No | Yes |
| HIST1H1T | 209 | 6.31 | 0.15 | No | Yes |
| HIST1H3G | 210 | 6.80 | 0.18 | No | Yes |
| ZRANB3 | 209 | 6.63 | 0.14 | No | Yes |
| LEO1 | 202 | 6.85 | 0.14 | No | No |
| SCRAM | 467 | 6.93 | 0.11 | N/A | N/A |
| GADD45A | 148 | 7.09 | 0.16 | No | No |
| SMARCB1 | 158 | 7.48 | 0.21 | No | No |
| INO80B | 124 | 7.79 | 0.23 | No | Yes |
Statistical significance of gene depletion EFC ratios, relative to the scrambled siRNA control, was determined by the two-tailed Bonferroni-corrected nonparametric Dunn’s test for p < 0.05.