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. 2020 Jun 15;31(13):1425–1436. doi: 10.1091/mbc.E20-02-0114

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© 2020 Rim et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — Loss of adaptor protein AP2α impairs clathrin-mediated cargo uptake but does not affect Wnt signal transduction. (A) CRISPR-mediated knockout of AP2α was confirmed via Western blot. Loss of AP2α impaired cellular uptake of fluorescently labeled transferrin (p < 0.001). Scale bar, 20 μm. (B) Change in nuclear and (C) cellular β-catenin upon stimulation with 5 nM Wnt3a was quantified in wild-type and AP2α knockout mESCs (wild type, n = 28; AP2α knockout, n = 23; no Wnt3a, n = 21). (D) Transcription of Axin2 upon stimulation with 1–5 nM Wnt3a was assayed in wild-type and AP2α knockout mESCs.