TABLE 3.
Plasmids.
| Plasmid Name | Description | Source |
|---|---|---|
| pDH22 | ARB1 in pRS316 | Dong et al. (2005) |
| pDH25.1 | UASG-UBI-R-FH-ARB1 in YCp111; LEU2, CEN | Dong et al. (2005) |
| p426Met25-FFL-GFP | UASMET25-FFL-GFP in pRS426; URA3, 2μ | Tkach and Glover (2008) |
| P416/Q103Addgene 1179 | UASTDH3-Htt-Q103-GFP in pRS416; URA3, CEN (actually Q97) | Krobitsch and Lindquist (2000) |
| P416/Q46(GC391)Addgene 1178 | UASTDH3-Htt-Q46-GFP in pRS416; URA3, CEN | Krobitsch and Lindquist (2000) |
| P416/Q25(GC390)Addgene 1177 | UASTDH3-Htt-Q25-GFP in pRS416; URA3, CEN | Krobitsch and Lindquist (2000) |
| isolated from Dharmacon strain | UASG PCYC1 GST-6xHis-ARB1 in pURA3; LEU2d, 2μ | Dharmacon Zhu et al. (2001) |
| pDH129 | FLAG-His6-ARB1 in YC1ac111; LEU2, CEN | Dong et al. (2005) |
| pRS425; pRS315 | LEU2, 2μ; LEU2, CEN | Christianson et al., (1992); Sikorski and Hieter (1989) |
| pRS313 Hsp104 K218T; K620TAddgene 1192 | HSP104 Double Walker A mutant; ATPase mutant (K218T and K620T); HIS3, CEN | Patino et al. (1996) |
| pRS416GAL-Hsp104A503V | HSP104 (A503V) in pRS416; URA3, CEN | Jackrel and Shorter (2014) |
| pADH1pr-MCS-ter | Sequences upstream and downstream of ADH1 (–371 to –12 and +1048–1236) separated by a polylinker in PCR2.1 | This study (S.S.) |
| pJF2140 | ADH1 fragment isolated from GC99 in pRS315; LEU2, CEN | This study (S.S.) |
| pSS2148 & pSS2175 | ADH1 fragment isolated from GC99 in pRS425; LEU2, 2μ using two different strategies | This study (S.S.) |
| pSOC4ΔSmaI | ADH1 (–371 to –12 and +1048–1236) fragment; URA3, 2μ | This study (D.L.W.) derivative of pSOC4 (gift of Williams and Cyert) |
| pXL-ABCF2-URA3 | PADH1 X. laevis ABCF2 cDNA in pSOC4ΔSmaI; URA3, 2μ | This study (D.L.W.) |
| pSS2182 | PADH1 X. laevis ABCF2; Kl LEU2, 2μ | This study (S.S.); derivative of pXL-ABCF2-URA3 |
| pSS2198, pSS2199 | PADH1 X.laevis ABCF2-K431W in pJF2182; LEU2 2μ (two clones constructed differently) | This study (SS) |
| pSS2125 | UASGC. elegans abcf-2 cDNA in pESC-LEU2; LEU2, 2μ | This study (S.S.); backbone from Agilent Technologies |
| pSS2174 | UASG C. elegans abcf-2-FLAG; LEU2, 2μ | This study (S.S.); derivative of pSS2125 |
| pSS2183 | PADH1 C. elegans abcf-2-FLAG; LEU2, 2μ | This study (S.S.); derivative of pSS2174 |
| pHS-ABCF3(high) | PADH1 human ABCF3 cDNA in pJF2148; LEU2 2μ | This study (A.G. and D.L.W.) |
| pHS-ABCF2(low) | PADH1 human ABCF2 cDNA in pJF2140; LEU2, CEN | This study (A.G. and D.L.W.) |
| pHS-ABCF3(low) | PADH1 human ABCF3 cDNA in pJF2140; LEU2, CEN | This study (A.G. and D.L.W.) |
| pHS-ABCF1(high) | PADH1 human ABCF1 in pJF2148; LEU2 2μ | This study (A.G. and D.L.W.) |
| pHS-ABCF1(low) | PADH1 human ABCF1 in pJF2140; LEU2, CEN | This study (A.G. and D.L.W.) |
| pBTP78 | C. elegans abcf-2 in PCR2.1 | This study (A.C. and B.Z.) |
| pBTP79 | C. elegans abcf-2 in pL4440 | This study (A.C. and B.Z.) |