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. 2020 Jun 1;31(12):1232–1245. doi: 10.1091/mbc.E20-02-0125

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© 2020 Marini et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Filament formation is independent of sfGFP tagging and overexpression of eIF2B. Average images of 20 tomographic slices of WT yeast cells showing (A) overexpressed eIF2B forming large bundles of filament in the cytoplasm and (B–F) endogenously expressed eIF2B forming smaller bundles of filaments. (B–D) White stars label autophagosomes, recognized as double-membrane vesicles containing mostly ribosomes. M = mitochondrion; N = nucleus; V = vacuole. Scale bars = 200 nm.