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. 2020 Jun 1;31(12):1232–1245. doi: 10.1091/mbc.E20-02-0125

Video S4.

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The fluorescent signal of eIF2B is distributed differently between stressed yeast cells and log‐phase growing cells. According to the fluorescent signal, the sfGFP‐labeled Gcn3 (α) subunit of eIF2B present a diffuse distribution throughout the cytoplasm in log‐phase growing and dividing cells. On the contrary, the fluorescence is concentrated into condensed foci‐like or elongated structures in stressed cells that have been energy depleted for 15 minutes at pH 5.5. Scale bar = 10 µm