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. 2020 Jun 16;10(6):1174. doi: 10.3390/nano10061174

Figure 4.

Figure 4

(AC), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide salt (MTT) assay of (A) bulk fosetyl-Al; (B) nanoFos; (C) CH-nanoFos. The viability measured as indicated in the Methods section represents the values as percentage of the respective control (untreated cells) ones from three independent experiments considered as 100%. Asterisks indicate significant values (p ˂ 0.05) from the respective untreated control cells. (D) optical microscopic (OM) micrographs show HaCat cells in culture. (a,b) untreated HaCat cells (100% of viability); (c) HaCat cells treated with bulk fosetyl-Al 1000 µg/mL; (d) HaCat cells treated with bulk fosetyl-Al 100 µg/mL; (e) HaCat cells treated with nanoFos 1000 µg/mL; (f) HaCat cells treated with nanoFos 100 µg/mL; (g) HaCat cells treated with CH-nanoFos 1000 µg/mL; (h) HaCat cells treated with CH-nanoFos 100 µg/mL. Bar = 20 µm.