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. 2020 Jun 22;12(6):1862. doi: 10.3390/nu12061862

Table 2.

List of primers used for murine intestine claudin expression experiments. Primer sequences used were either published sequences or designed by Dr Rajagopal. The compiled list was kindly supply by Professor A Yu of the University of Kansas Medical Center.

Gene Nucleotide Accession Number Forward Primer Reverse Primer Reference
Claudin 1 NM_016674 CCTTCGGGAGCTCAGGTGCG CCGCGTTGGCCATGGCTCTT [31]
Claudin 2 NM_016675 TGGCGTCCAACTGGTGGGCT ACCGCCGTCACAATGCTGGC [31]
Claudin 3 NM_009902 GGGCAGTCTCTGTGCGAGCC CGGACGTCTGTCGCCGGGAA [31]
Claudin 4 BC132376 TGGGGACAGGCAAACCCGGA CTTGCCGGCCGTAAGGAGCC [31]
Claudin 5 NM_013805 GCTCAGTGCACCACCTGCGT GAACCAGCAGAGCGGCACGA [31]
Claudin 6 NM_018777 AGCACTCGCCCCCTCAACCTC CATGGGCAGGGCACAGGACAC [31]
Claudin 7 NM_001193619 CACGCAGAGCACCGGCATGA AGGGCGAGCACCGAGTCGTA Blast
Claudin 8 NM_018778 TCCCTGTCAGCTGGGTTGCCA GCTCGCGCTTTAGGGCCACA [31]
Claudin 9 NM_020293 TCCCAAGTGGCACCTCACGGT CGCGTTCCTCTCTGCTGGCTG [32]
Claudin 10a NM_023878 GTGGCAGCAGGCAAGGCTGA CACAGACGACGCTCGGGTGG Blast
Claudin 10b NM_001160099 CTCCATCTCGGGCTGGGTGC CAACGCCAGCATGGAGGGGA Blast
Claudin 11 NM_008770 TGGTTCCAGCTCGCCAACGC TTACAGCACCTCGGCGGGCA [32]
Claudin 12 NM_001193659 AGGTATTCCCGAGCGGAGCCA CCCGGAGGCTTCAGGGAACCA [32]
Claudin 13 NM_020504 TGACTCGTCCTGGTCCTGCCA GGTCACCCTCCAAACGGGCA [32]
Claudin 14 NM_001165926 GCAGCTGCGGCAAAGGAGTCT ACGGCCGTCTAATGGGTCCCT [32]
Claudin 15 NM_021719 TATGAACTGGGCCCCGCCCT ATCCGAGGTGGCACGGGGTA [32]
Claudin 16 NM_053241 CCACGAACCAGGATGTGCCCG GCGAGGGTCGTGGAGGTCAC [32]
Claudin 17 NM_181490 CTCCAGCGAGAGGGTCAAAG AGCAGCAATATCCGCAGAGC [32]
Claudin 18 NM_001194921 CCTGACACCAGATGACAGCA GGCAACATTTTGGCCAGAGG [32]
Claudin 19 NM_153105 CAGAGCCGGAGAGGGCGAACA TCTGGGCAAGAGGGTTGCTGG [32]
Claudin 20 NM_001101560 GCACTCTAAAATACTCCATTC TGAAGCAGACTCCTCCAGC Blast
Claudin 21 CTGGGACTATTGGGACTTCTG AGGAGACTGGAAAGAGGGTAG [33]
Claudin 22 NM_029383 TTCCGAACGGCAACGCAGGC CCCATCCCAGCAGGGAGAGCA Blast
Claudin 23 NM_027998 CGACGGACAGCATCGGCCTC GGACTTGGGTGGCGGTCGTG Blast
Claudin 24 NM_001111318 GAACGGCCATGCAATCAGTAGGGC GACGCAGGATTTCCAGAGCCCC Blast
Claudin 25 NM_171826 GAGAGGATGGGCGTATGCAG ACTGCTCCAAGATGCTACGG Blast
Claudin 26 NM_029070 GTGCGGGTGGGATCGCGTAA CCCACGCTCCCCGTCTGTTC Blast
Claudin 27 NM_001085535 TGGGTAGCCGGTGCCTCGAA GCAGGCACCTAGCACAGGGG [33]
β-actin NM_007393 ATATCGCTGCGCTGGTCGTC AGGATGGCGTGAGGGAGAGC Blast