Figure 3.

(A) Mean values and standard deviation of sirtuin-1 activity levels in the gastrocnemius muscle of the different study groups of mice, as measured by fluorescence in arbitrary units (a.u.). Definition of abbreviations: a.u., arbitrary units; I, immobilization; R, recovery; Res, resveratrol; Cur, Curcumin. p = 0.116: statistical significance between 7dR + Cur versus 7dR. The effect of immobilization and treatment and interaction effects are also indicated as actual p values for each variable. (B) Mean values and standard deviation of sirtuin-1 protein content in the gastrocnemius muscle of the different study groups of mice, as measured by optical densities in arbitrary units (OD, a.u.). Definition of abbreviations: OD, optical densities; a.u., arbitrary units; I, immobilization; R, recovery; Res, resveratrol; Cur, curcumin. Statistical significance is represented as follows: *, p < 0.05 between 7dI animals and non-immobilized mice; §, p < 0.05 between the 7dI and 7dR groups of animals; ^#, p < 0.05 for any group of pharmacologically treated mice (resveratrol or curcumin) compared to their respective group (7dI or 7dR mice). The effect of immobilization and treatment and interaction effects are also indicated as actual p values for each variable. (C) Representative immunoblots of sirtuin-1 and GAPDH proteins in the gastrocnemius muscle of all study groups of mice. Arrowheads indicate the corresponding analyzed band. Definition of abbreviations: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MW, molecular weight; kDa, kilodalton; I, immobilization; R, recovery; Res, resveratrol; Cur, curcumin.