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. 2020 Jun 23;12(6):1870. doi: 10.3390/nu12061870

Figure 5.

Figure 5

(A) Representative images of immunofluorescence staining of DAPI (left panels), Pax-7 (middle-left panels), Myf-5 (middle panels) and cells positively stained for both Pax-7 and Myf-5 markers (middle-right panels), and negative control (right panels) in the gastrocnemius muscle of the non-immobilized mice and immobilized mice with and without treatment (7dI, 7dI + Res and 7dI + Cur groups of mice). Thin arrows indicate Pax-7-positive cells, and arrowheads indicate double-stained nuclei for both Pax-7- and Myf-5-positive cells (activated satellite cells). Definition of abbreviations: Pax-7, paired box-7; Myf-5, myogenic factor 5; DAPI, 4’,6-diamino-2-fenilindol; I, immobilization; Res, resveratrol; Cur, curcumin. (B) Representative images of immunofluorescence staining of DAPI (left panels), Pax-7 (middle-left panels), Myf-5 ((middle panels) and cells positively stained for both Pax-7 and Myf-5 markers (middle-right panels) and negative control (right panels) in the gastrocnemius muscle of the recovery mice with and without treatment (7dR, 7dR + Res and 7dR + Cur groups of mice). Thin arrows indicate Pax-7-positive cells, and arrowheads indicate double-stained nuclei for both Pax-7- and Myf-5-positive cells (activated satellite cells). Definition of abbreviations: Pax-7, paired box-7; Myf-5, myogenic factor 5; DAPI, 4’,6-diamino-2-fenilindol; R, recovery; Res, resveratrol; Cur, curcumin.