Table 4.
Hematic parameters throughout the study: values of cholesterol, triglyceride and glucose from Day 47–Day 84; glucose tolerance test on Day 84. Group 1: standard diet + vehicle; group 2: HFD + vehicle; group 3: HFD + AP-NHm 1 co-treatment; group 4: HFD + AP-NHm 2 co-treatment; group 5: HFD + AP-NHm 1 post-injury; group 6: HFD + AP-NHm 2 post-injury.
| Hematic total cholesterol (mg/dL) | Hematic HDL (mg/dL) |
Hematic LDL (mg/dL) |
||||||||||
| Group | Day 49 | Day 56 | Day 63 | Day 70 | Day 77 | Day 84 | Day 84 | Day 84 | ||||
| 1 | 102.6 ± 5.1 | 105.0 ± 5.0 | 107.0 ± 1.2 | 105.3 ± 0.9 | 102.6 ± 2.1 | 103.8 ± 4.1 | 51.8 ± 8.5 | 43.8 ± 6.7 | ||||
| 2 | 140.7 ± 3.4 ** | 145.8 ± 3.8 ** | 149.9 ± 2.6 ** | 155.3 ± 4.8 ** | 153.8 ± 5.3 ** | 160.6 ± 3.5 ** | 60.3 ± 7.0 | 73.8 ± 5.2 ** | ||||
| 3 | 135.6 ± 4.9 | 142.8 ± 5.1 | 145.3 ± 3.7 | 144.2 ± 4.6 ^ | 143.1 ± 3.1 ^ | 150.6 ± 6.8 | 62.2 ± 5.2 | 70.6 ± 3.3 | ||||
| 4 | 103.3 ± 3.2 ^^ | 104.0 ± 4.3 ^^ | 113.0 ± 5.4 ^^ | 120.0 ± 6.2 ^^ | 125.0 ± 4.8 ^^ | 129.1 ± 4.6 ^^ | 73.4 ± 2.3 ^ | 36.9 ± 6.3 ^^ | ||||
| 5 | 142.5 ± 6.2 | 146.4 ± 6.2 | 147.3 ± 6.5 | 145.1 ± 8,3 | 150.3 ± 4.1 | 156.9 ± 8.6 | 59.1 ± 10.5 | 46.0 ± 9.4 ^^ | ||||
| 6 | 141.9 ± 6.1 | 144.9 ± 5.3 | 143.8 ± 3.3 | 144.1 ± 4.1 ^ | 142.8 ± 3.2 ^ | 140.1 ± 5.4 ^^ | 62.9 ± 4.9 | 53.2 ± 4.0 ^^ | ||||
| Triglyceride hematic levels (mg/dL) | ||||||||||||
| Group | Day 49 | Day 56 | Day 63 | Day 70 | Day 77 | Day 84 | ||||||
| 1 | 69.0 ± 6.9 | 71.6 ± 4.4 | 72.3 ± 6.4 | 70.5 ± 3.8 | 69.9 ± 6.1 | 72.9 ± 5.2 | ||||||
| 2 | 142.3 ± 4.3** | 145.7 ± 6.2 ** | 146.3 ± 4.8 ** | 151.2 ± 2.2 ** | 154.4 ± 5.0 ** | 159.3 ± 7.7 ** | ||||||
| 3 | 115.6 ± 4.3^ | 114.1 ± 6.6 ^ | 109.0 ± 5.7 ^^ | 115.5 ± 3.1 ^ | 105.6 ± 4.3 ^ | 100 ± 5.8 ^^ | ||||||
| 4 | 85.8 ± 5.0^ | 84.2 ± 6.4 ^ | 86.5 ± 10.7 ^ | 90.4 ± 7.8 ^^ | 89.5 ± 5.1 ^^ | 92.7 ± 5.3 ^^ | ||||||
| 5 | 145.4 ± 7.6 | 147.1 ± 9.5 | 143.7 ± 6.7 | 140.8 ± 6.1 | 141.2 ± 3.6 ^ | 139.8 ± 4.4 ^^ | ||||||
| 6 | 142.0 ± 9.0 | 145.7 ± 5.5 | 140.9 ± 6.4 | 130.1 ± 3.2 ^ | 128.6 ± 5.0 ^^ | 129.2 ± 3.0 ^^ | ||||||
| Hematic glucose (mg/dL) | ||||||||||||
| Group | Day 49 | Day 56 | Day 63 | Day 70 | Day 77 | Day 84 | ||||||
| 1 | 103.0 ± 2.9 | 105.7 ± 4.4 | 101.3 ± 10.1 | 103.3 ± 4.4 | 99.6 ± 6.1 | 105.3 ± 4.6 | ||||||
| 2 | 160.5 ± 6.9 ** | 155.4 ± 4.2 ** | 158.3 ± 3.9 ** | 162.5 ± 6.1 ** | 164.4 ± 5.1 ** | 168.3 ± 9.4 ** | ||||||
| 3 | 160.6 ± 8.0 | 158.3 ± 6.1 | 163.5 ± 10.7 | 161.5 ± 5.1 | 155.0 ± 2.8 | 166.7 ± 6.5 | ||||||
| 4 | 123.0 ± 5.1^ | 120.4 ± 5.5 ^ | 123.9 ± 2.0 ^ | 133.8 ± 6.0 ^ | 139.4 ± 6.1 ^ | 145.9 ± 6.4 ^ | ||||||
| 5 | 152.0 ± 9.9 | 158.3 ± 6.5 | 163.3 ± 5.4 | 159.6 ± 5.9 | 161.2 ± 8.8 | 156.3 ± 5.7 | ||||||
| 6 | 153.0 ± 6.2 | 158.5 ± 8.1 | 154.8 ± 7.1 | 146.3 ± 5.5 ^ | 144.4 ± 3.9 ^ | 143.8 ± 4.0 ^ | ||||||
| Glucose tolerance test (Day 84): hematic glucose (mg/mL) | ||||||||||||
| Group | Pretest | 15 min | 30 min | |||||||||
| 1 | 105.3 ± 4.6 | 161.7 ± 3.2 | 189.0 ± 9.0 | |||||||||
| 2 | 168.3 ± 9.4 ** | 267.0 ± 6.7 ** | 305.0 ± 5.9 ** | |||||||||
| 3 | 166.7 ± 6.5 | 264.3 ± 8.5 | 291.2 ± 6.2 | |||||||||
| 4 | 145.9 ± 6.4 ^ | 182.4 ± 9.0 ^ | 221.8 ± 11.4 ^ | |||||||||
| 5 | 156.3 ± 5.7 | 263.7 ± 10.2 | 294.7 ± 9.9 | |||||||||
| 6 | 143.8 ± 4.0 ^ | 220.8 ± 10.1 ^ | 259.1 ± 11.6 ^ | |||||||||
Each value represents the mean ± SEM of 12 mice per group. ** p < 0.01 vs standard diet + vehicle, ^ p < 0.05 vs HFD + vehicle, ^^ p < 0.01 vs HFD + vehicle. HFD: high-fat diet.