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. 2020 Jun 17;10(6):1183. doi: 10.3390/nano10061183

Figure 6.

Figure 6

(A) Scheme of ICOVIR15Luc encoded by pLR1 plasmid. qPCR indicates the location of the target sequence for the quantitative polymerase chain reaction on the Ad5 hexon gene, present within late transcription unit 3 (L3). LITR: left inverted terminal repeat; E2F boxes: locations for E2F transcription factors; E1A: early transcription unit 1; MLP: major late promotor; L1–L5: late transcription units; SA: splicing acceptor; K: replacement of KKTK motif with RGDK; pA: polyadenylation signal sequence; RITR: right inverted terminal repeat. (B) Graph represents RNA copies of pLR1 (black bars) at different days after transfection of SW480 cells with PEI-AuNPs/pLR1 at 20 µg/mL of pLR1. RNA was measured from cell lysates and results were plotted on a standard curve prepared with known amounts of the same plasmid. White bars (plotted on right Y axis) represent luminescence at different days of samples from the SW480 cells transfected with PEI-AuNPs/pLR1 at 20 µg/mL of pLR1.