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. 2020 May 1;8(2):21. doi: 10.3390/medsci8020021

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell rounding during cultivation. (A) Freshly isolated cardiomyocyte with phalloidin staining of actin to visualize striation of sarcomeres. (B) Cardiomyocytes after 48 h cultivation in the presence of fetal calf serum, indicating sarcomere degradation from the cell poles. (C) Quantification of the number of rod-shaped cardiomyocytes and round cardiomyocytes at start of cultivation (day 0) and at day 2. Data show the effect of serum on cell rounding. Data are means ± SD from n = 8 preparations (237–410 cells per preparation were analyzed).