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Protein expression in cultured cardiomyocytes within 48 h in the presence or absence of fetal calf serum (FCS). (A) mRNA expression of proteins of interest (n = 6). (B) Representative western blot of LMP-2, myosin heavy chain, and β-actin. The double band of LMP-2 in B indicates activation of LMP-2 and incorporation into proteasomes. (C) Quantitative analysis of the western blot shown in B. Data are means ± SD. (*, p < 0.05 vs. FCS free)
