Table 1.

Cytotoxic and antiproliferative activities of the 2,5-DHBA–gelatin conjugate.

Substance	Concentration, µg/mL	Cytotoxicity (%) (a)		Antiproliferative Activity (%) (a)
		HT1080	A-172	HT1080	A-172
2,5-DHBA–gelatin	1000	104 ± 15	97 ± 12	102 ± 16	102 ± 13
	300	96 ± 12	96 ± 14	99 ± 14	101 ± 12
	100	103 ± 16	100 ± 13	104 ± 17	94 ± 17
	30	95 ± 13	93 ± 14	96 ± 17	96 ± 16
	10	97 ± 15	99 ± 16	93 ± 14	96 ± 15
Heparin	1000	104 ± 17	97 ± 14	95 ± 15	92 ± 15
	300	101 ± 13	99 ± 14	94 ± 12	98 ± 14
	100	101 ± 12	105 ± 15	99 ± 13	98 ± 14
	30	93 ± 14	96 ± 15	103 ± 15	102 ± 16
	10	98 ± 13	94 ± 13	101 ± 15	95 ± 11
Chondroitin sulfate	1000	100 ± 16	99 ± 12	91 ± 12	94 ± 16
	300	100 ± 12	103 ± 13	95 ± 14	92 ± 15
	100	104 ± 16	105 ± 13	93 ± 12	95 ± 15
	30	94 ± 14	101 ± 16	99 ± 12	100 ± 13
	10	99 ± 16	95 ± 16	100 ± 13	101 ± 13
Dermatan sulfate	1000	99 ± 17	102 ± 15	92 ± 13	91 ± 15
	300	103 ± 14	97 ± 13	96 ± 14	93 ± 14
	100	104 ± 18	100 ± 14	101 ± 13	95 ± 14
	30	101 ± 16	101 ± 15	95 ± 14	104 ± 14
	10	97 ± 14	96 ± 14	103 ± 15	107 ± 17
Geldanamycin (b)	5.6	5 ± 3	0	0	0
	0.56	43 ± 9	3 ± 3	13 ± 7	0
	0.056	94 ± 11	13 ± 4	55 ± 12	2 ± 2
	0.0056	98 ± 15	78 ± 14	100 ± 16	57 ± 8

^(a) The mean OD₅₅₀ values for cells treated with different substances relative to control untreated cells, expressed as percentage, are presented. The mean OD₅₅₀ value of control untreated cells was taken as 100%. The mean values of four to six repeats ± SD are presented. ^(b) Geldanamycin, which exhibits cytotoxic and antiproliferative activities, was used as a positive control.