Table 2.

Real-time PCR conditions for HaCaT cells.

Gene	Primer Sequence	Conditions	Size (bp)
IL-1 α	5′-CATGTCAAATTTCACTGCTTCATCC-3′	5 s at 95 °C, 8 s at 55 °C,	421
	5′-GTCTCTGAATCAGAAATCCTTCTATC-3′	17 s at 72 °C for 45 cycles
IL-1 β	5′-GCATCCAGCTACGAATCTCC-3′	5 s at 95 °C, 14 s at 58 °C,	708
	5′-CCACATTCAGCACAGGACTC-3′	28 s at 72 °C for 40 cycles
TNF-α	5′-CAGAGGGAAGAGTTCCCCAG-3′	5 s at 95 °C, 6 s at 57 °C,	324
	5′-CCTTGGTCTGGTAGGAGACG-3′	13 s at 72 °C for 40 cycles
IL-6	5′-ATGAACTCCTTCTCCACAAGCGC-3′	5 s at 95 °C, 13 s at 56 °C,	628
	5′-GAAGAGCCCTCAGGCTGGACTG-3′	25 s at 72 °C for 40 cycles
IL-8	5-ATGACTTCCAAGCTGGCCGTG-3′	5 s at 94 °C, 6 s at 55 °C,	297
	5-TGAATTCTCAGCCCTCTTCAAAAACTTCTC-3′	12 s at 72 °C for 40 cycles
TGF-β	5′-CCGACTACTACGCCAAGGAGGTCAC-3′	5 s at 94 °C, 9 s at 60 °C,	439
	5′-AGGCCGGTTCATGCCATGAATGGTG-3′	18 s at 72 °C for 40 cycles