Figure 1.

Degree of hydrolysis (DH) or digestibility of the protein or its hydrolysates (A) and size exclusion chromatograms (SECs) of the enzyme blank, which was the digesta of thermally inactivated Alcalase (A₀-GID), 10-hydrolysate (H₁₀), and its digesta (H₁₀-GID) (B). PD, protein digesta; GD, gastric digesta; and GIDs, GI digesta. The different lowercase and uppercase letters and numbers indicated the differences in the mean values of the hydrolysate, GDs, and GIDs, respectively (p < 0.05). Elution volume (V_e) at 13.20, 18.10, and 20.77 mL was used to define the M_W distribution of the peptides, as detailed in Table 2.