Table 1.
Alpha-amino group content and chemical antioxidant activities of enzyme blanks 1.
| Samples | A 0 | Pep-I | Pep-Pan-I | A0-GID | H10-GID# | |
|---|---|---|---|---|---|---|
| Parameters | ||||||
|
α-Amino (mmol L-Leu) |
Free | 0.02 ± 0.00 a | 0.01 ± 0.00 a | 1.05 ± 0.01 b | 1.02 ± 0.02 b | 2.69 ± 0.09 c |
| Total | – | – | – | 2.00 ± 0.15 a | 4.73 ± 0.12 b | |
| TEAC (μmol Trolox) | 12.18 ± 0.98 a | 39.02 ± 0.00 b | 389.54 ± 0.55 c | 487.47 ± 0.65 d | 1004.83 ± 7.35 e | |
| FRAP (μmol Trolox) | 0.11 ± 0.08 a | 0.03 ± 0.00 a | 1.94 ± 0.04 c | 1.78 ± 0.04 b | 4.64 ± 0.04 d | |
| FICC (μmol EDTA) | 5.99 ± 0.40 a | ND | 7.33 ± 0.68 ab | 11.59 ± 2.11 c | 8.25 ± 0.35 b | |
| ORAC (μmol Trolox) | 12.39 ± 1.09 a | 0.57 ± 0.03 a | 235.69 ± 58.40 b | 370.63 ± 10.35 c | 984.54 ± 34.18 d | |
| (μmol GSH) | ND | ND | 0.59 ± 0.05 a | 1.06 ± 0.16 b | 3.95 ± 0.19 c | |
1 The results were calculated as the sample that was brought to 25 mL. a–e Different lowercase letters in the same row indicated the differences in mean values (p < 0.05). # Without enzyme blank subtraction. ‘–’, the values were not determined; ND, the values were not detected; A0, thermally-inactivated Alcalase; Pep-I, blank of gastric (pepsin) digestion; Pep-Pan-I, blank of GI (pepsin and pancreatin) digestion; A0-GID, GI digesta of A0; and H10-GID, GI digesta of 10-h hydrolysate.