Table 2.

Molecular weight (MW) distribution of protein hydrolysates, digesta, and enzyme blanks ¹.

Sample	Percentage of Area under Chromatogram
	>1250 Da	1250–330 Da	330–220 Da	<220 Da
Protein hydrolysates
H 2	12.29 ± 1.31 c	77.49 ± 1.67 b	10.00 ± 0.36 a	0.22 ± 0.12 a
H 6	7.60 ± 0.68 b	75.64 ± 1.88 ab	15.59 ± 0.57 b	1.17 ± 0.76 ab
H 10	4.46 ± 0.30 a	75.55 ± 0.92 ab	18.73 ± 0.70 c	1.25 ± 0.39 ab
H 16	3.61 ± 0.54 a	73.52 ± 0.34 a	21.02 ± 0.75 d	1.85 ± 0.15 b
Digesta of protein hydrolysates
P-GID	5.79 ± 0.30 A	37.50 ± 0.27 BCD	35.27 ± 0.37 BC	21.44 ± 0.36 D
H2-GID	6.13 ± 0.11 A	37.74 ± 0.38 CD	35.70 ± 0.32 BC	20.43 ± 0.55 CD
H6-GID	5.71 ± 0.21 A	38.77 ± 0.61 D	36.24 ± 0.16 BC	19.28 ± 0.27 ABC
H10-GID	5.76 ± 0.24 A	37.59 ± 0.16 BCD	36.61 ± 0.30 C	20.04 ± 0.27 BC
H16-GID	6.07 ± 0.45 A	37.52 ± 0.26 BCD	36.59 ± 0.46 C	19.82 ± 0.24 BC
Enzyme blanks
Pan 0	16.47 ± 0.34 D	36.57 ± 0.49 BC	28.90 ± 0.96 A	18.06 ± 0.69 A
A0-Pep0-Pan0	14.96 ± 1.08 C	36.32 ± 0.11 B	29.83 ± 1.40 A	18.90 ± 0.42 AB
A0-GID	8.99 ± 0.43 B	26.87 ± 0.74 A	34.57 ± 0.64 B	29.56 ± 0.71 E

¹ MW at elution volume (V_e) 13.20, 18.10 and 20.77 mL were calculated by formula LogMW = −0.649ln (K_av) + 2.1656 R² = 0.988, respectively, and used to define the MW distribution of peptides. ^a–d Different lowercase letters indicate differences in mean values among protein hydrolysates (p < 0.05). ^A–D Different uppercase letters indicate differences in mean values among digesta and enzyme blanks (p < 0.05). A₀, Pep₀, and Pan₀ were referred to as thermally-inactivated Alcalase, pepsin, and pancreatin, respectively; A₀-Pep₀-Pan₀, the mixture of A₀, Pep₀, and Pan₀; and A₀-GID, digesta of A₀.