Table 2.
The approximate composition, surface hydrophobicity, emulsion and thermal properties of thraustochytrid protein isolates (MAN65 and MAN70) extracted at pH 12.0 and 25 °C.
| TPI Characteristics | MAN65 | MAN70 |
|---|---|---|
| Approximate Composition | ||
| Moisture (%) | 3.13 ± 0.85 | 4.30 ± 0.61 |
| Protein (%) | 91.64 ± 0.45 | 89.08 ± 0.79 |
| Ash (%) | 0.035 ± 0.12 | 3.65 ± 0.06 |
| Lipid (%) | 3.05 ± 0.07 | 3.13 ± 0.02 |
| Surface hydrophobicity | 53.33 ± 0.27 | 60.85 ± 0.93 |
| Emulsion | ||
| Emulsifying activity index (m2/g) | 693.98 ± 2.83 | 784.12 ± 1.82 |
| Emulsion stability index (min) | 192.09 ± 1.75 | 209.86 ± 12.53 |
| Secondary Structure | ||
| Alpha helix (%) | 10.00 ± 0.20 | 15.00 ± 0.10 |
| Beta sheet (%) | 34.00 ± 0.10 | 29.00 ± 0.20 |
| Random coil (%) | 55.00 ± 0.05 | 57.00 ± 0.05 |
| Thermal Parameters | ||
| Denaturation temperature (Td) (°C) | 167.80 ± 0.50 | 174.50 ± 0.20 |
| Denaturation enthalpy (ΔH) (°C) | 3.33 ± 0.40 | 3.21 ± 0.40 |
| Initial decomposition temperature (IDT) (°C) | 233.00 ± 3.40 | 242.00 ± 1.60 |
| Temperature 50 wt% decomposition (TD1/2) (°C) | 232.96 ± 3.39 | 242.04 ± 1.56 |
| Temperature of maximum of decomposition occurs (MDT) (°C) | 342.40 ± 2.20 | 345.6 ± 0.30 |