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. 2020 May 27;10(6):1028. doi: 10.3390/nano10061028

Figure 7.

Figure 7

Z-stack images showing nanoMIL-89 uptake in PAECs. Orthogonal and 3D projections of Z-stack images of pre-stained PAECs showing: Untreated (control) PAECs and PAECs treated with 10 µg/mL nanoMIL-89. Projections are: (a) Transverse (xz, blue), (b) Sagittal (yz, red), (c) Frontal (xy, green), (d) 3D projection of Z-stack images and (e) 3D volume rendering of a single cell with a sliced view showing the cell content. Channels used—Red: CellTrackerTM Red CMTPX Dye, Green: phalloidin, Blue: diamidino-2-phenylindole (DAPI), and phase contrast: nanoMIL-89. Images indicate that nanoMIL-89 are internalized within the cytoplasm of the cells (black circle). Scale bar is 25 µm.