Table 2.
Chromatographic and spectroscopic characteristics in the HPLC eluent of chlorophyll pigments.
| Pigments | Peak | tr 1 | kc’ 2 | Spectroscopic Characteristics | |
|---|---|---|---|---|---|
| Absorption Maxima (nm) | |||||
| No | Soret | Q 3 | |||
| Series a | |||||
| Pheophorbide a | 1 | 7.6 | 2.7 | 410 | 666 |
| Pyropheophorbide a | 2 | 8.9 | 3.3 | 410 | 666 |
| Mg-152-Me-phytyl-chlorin e6 ester | 5 | 14.9 | 6.2 | 416 | 638 |
| 152-Me-phytyl-chlorin e6 ester | 6 | 15.1 | 6.3 | 400 | 662 |
| Chlorophyll a | 9 | 18.8 | 8.1 | 432 | 666 |
| Chlorophyll a’ | 9’ | 19.6 | 8.5 | 432 | 666 |
| 132-OH-chlorophyll a | 10 | 20.2 | 8.8 | 434 | 664 |
| Mg-152-Me-phytyl-isochlorin e4 ester | 11 | 21.0 | 9.2 | 416 | 638 |
| 152-Me-phytyl-isochlorin e4 ester | 13 | 26.1 | 11.7 | 400 | 662 |
| Pheophytin a | 14 | 26.3 | 11.8 | 410 | 666 |
| Pheophytin a’ | 14’ | 26.8 | 12.0 | 410 | 666 |
| Pyropheophytin a | 15 | 29.6 | 13.4 | 410 | 666 |
| Series b | |||||
| Mg-152-Me-phytyl-rhodin g7 ester | 3 | 13.1 | 5.4 | 450 | 626 |
| 152-Me-phytyl-rhodin g7 ester | 4 | 14.0 | 5.8 | 426 | 650 |
| Chlorophyll b | 7 | 16.4 | 7.0 | 466 | 650 |
| Chlorophyll b’ | 7’ | 16.9 | 7.2 | 466 | 650 |
| 132-OH-chlorophyll b | 8 | 17.6 | 7.5 | 466 | 646 |
| Pheophytin b | 12 | 24.0 | 10.7 | 436 | 654 |
1 tr: Retention time (min); 2 kc’: Retention factor = (tr − tm)/tm where tm is the retention time of an unretained component; 3 Q: maximum in the red region of the spectrum.