Table 3.
Antibodies used for double-labeling immunofluorescence histochemistry
| Combination | Primary antibody | Dilution | Vendor | Secondary antibody | Dilution | Vendor |
|---|---|---|---|---|---|---|
| CCL2+BLBP | Rabbit anti-CCL2 | 1:200 | Biorbyt, CA | Bio-Horse anti-Rabbit IgG + TSA Fluorescein | 1:100 | Vector, CA |
| Mouse anti-BLBP | 1:200 | Abcam, MA | Cy3-Donkey anti-Mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories. Inc, PA | |
| CCL2+NeuN | Rabbit anti-CCL2 | 1:200 | Biorbyt, CA | Bio-Horse anti-Rabbit IgG + TSA Fluorescein | 1:100 | Vector, CA |
| Mouse anti-NeuN | 1:100 | EMD Millipore, MA | Cy3-Donkey anti-Mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories. Inc, PA | |
| CCL2+Iba-1 | Rabbit anti-CCL2 | 1:200 | Biorbyt, CA | Bio-Horse anti-Rabbit IgG + TSA Fluorescein | 1:100 | Vector, CA |
| Goat anti-Iba-1 | 1:200 | Abcam, MA | Cy3-Donkey anti-Goat IgG | 1:100 | Jackson ImmunoResearch Laboratories. Inc, PA | |
| MCH+BLBP | Rabbit anti-MCH | 1:1000 | Phoenix Pharmaceuticals, CA | FITC-Donkey anti-Rabbit IgG | 1:50 | Jackson ImmunoResearch Laboratories. Inc, PA |
| Mouse anti-BLBP | 1:200 | Abcam, MA | Cy3-Donkey anti-Mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories. Inc, PA |
CCL2 C-C motif ligand 2, BLBP radial glia marker brain lipid-binding protein, NeuN neuronal marker, Iba-1 microglia marker, MCH melanin-concentrating hormone