Table 8.

Effects of ethanol on MCH and BLBP cells and processes in NEP and mHYP of female embryos

Labeled cells/processes	Location	Control (cells, objects/μm2)	Ethanol (cells, objects/μm2)
MCH+	NEP	3.77E− 6 ± 2.47E− 6	2.58E− 5 ± 2.18E− 6*
	mHYP	4.21E− 5 ± 1.89E− 6	4.92E− 5 ± 2.31E− 6*
BLBP+	NEP	1.09E− 4 ± 7.76E− 6	1.31E− 4 ± 6.44E− 6*
	mHYP	1.37E− 4 ± 6.99E− 6	1.65E− 4 ± 8.69E− 6*

Maternal administration of ethanol (2 g/kg/day, E10–E15) was compared to isocaloric maltose-dextrin control solution (Control) group in the NEP and mHYP areas of female embryos, measured using DIG-labeled in situ hybridization and immunofluorescence histochemistry. Data are mean ± SEM. n = 6/group, *p < 0.05 versus Control. One-way ANOVA was used to compare group means followed by SDS post hoc test.

BLBP brain lipid-binding protein, MCH melanin-concentrating hormone, mHYP medial hypothalamus, NEP hypothalamic neuroepithelium