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. 2020 Jul 11;39:131. doi: 10.1186/s13046-020-01610-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — HOTAIR is upregulated in EC tissues and cell lines. a HOTAIR expression analyzed using the TCGA database. b HOTAIR expression in EC tissues (n = 70) and adjacent normal tissues (n = 70) determined by RT-qPCR, normalized to GAPDH. (C) HOTAIR expression in EC cell lines and HEEC determined by RT-qPCR, normalized to GAPDH. d The localization of lncRNA HOTAIR in TE-1 EC cells predicted using the subcellular location website (http://lncatlas.crg.eu/). e The localization of lncRNA HOTAIR in TE-1 EC cells verified using FISH assay (× 200). Values obtained from three independent experiments in triplicate are expressed as mean ± SD and analyzed using paired t test between EC tissues and adjacent normal tissues, and using unpaired t test between EC cells and HEEC cells. In panel b, * represents comparison with adjacent normal tissues, * p < 0.05. In panel c, * represents comparison with HEEC cells, * p < 0.05