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. 2020 Jul 11;19:140. doi: 10.1186/s12934-020-01397-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Hydrolytic activity of Mg-αGlu (red bars) and Mr-αGlu (black bars) on the referred substrates. All reactions were incubated for 20 min at 30 °C and pH 7.0. After inactivation at 80 °C, released glucose was determined using the GOD-POD colorimetric assay. Activity towards pNPG was determined by the measurement of the free pNP in the reaction. The values obtained are the average of three independent experiments. Standard errors are indicated