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. 2020 Jul 11;19:140. doi: 10.1186/s12934-020-01397-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Representative HPAEC-PAD chromatogram of transglucosylation products synthesised in overload sucrose reactions. Chromatograms correspond to the sugar mixtures obtained by using M. reukaufii cell extract (blue line), Mr-αGlu (black line) or Mg-αGlu (red line) for about 71–76% sucrose conversion. Peaks assignation: (1) glucose, (2) fructose, (3) sucrose, (4) trehalulose, (5) melezitose, (6) isomelezitose, (7) theanderose, (8) erlose, (9) esculose, (*) non-identified peak (a). Chemical structures of the oligosaccharides synthesised by Metschnikowia yeasts α-glucosidases. The glucose unit transferred to free fructose or sucrose is represented in red (b). Molar ratio of glucose transferred to trehalulose or isomelezitose related to the total initial glucose. Reactions using different initial concentrations of sucrose plus fructose or sucrose (control reactions) were performed in triplicate. Standard errors are indicated (c)