Table 2.

Primers used in this study

Primer	Sequence
Mr-αGluF	ATGACTGACGCTATTTGGTGG
Mr-αGluR	CTAGTTCTTGACAATGTACAATCTACTCTC
RFpET28F	CCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGACTGACGCTATTTGGTGGAAAG
RFpET28R	GATCTCAGTGGTGGTGGTGGTGGTGGTTCTTGACAATGTACAATCTACTCTCG
D206AF	TCAGAATCGCGACTGCTGGTATGTACTCCAAGGTGCAAACCTTTGAGG
D206AR	ACCAGCAGTCGCGATTCTGAAACCGTCCACACCCTTCTCGAACCAG
E264QF	ACTGTTGGTCAGGTGGGCCACTCTTCTCGTGAAGATGCCTTGAAGTACGTC
E264QR	GTGGCCCACCTGACCAACAGTCATGGCGTCGTACTTGGAGGTCACCTTC
D339AF	GAGAACCACGCGCAACCAAGATGTATCACTCGTTTCGGTAACGACAGCCCAG
D339AR	CATCTTGGTTGCGCGTGGTTCTCGAAGAAACAAGTTGACCATGCATCAGTTCC

Sequences complementary to the insertion site in the pET28b(+) plasmid are underlined. Initial ATG and TAG stop codons are indicated in bold