Fig. 5.

HOTAIR OE induced c-Met inhibition decreases individual motility and invasion ability of SNU-449 cells. Real-time (by xCELLigence RTCA) and end-point (cell culture inserts) analysis of motility and invasion of MOCK and HOTAIR OE cell clones. Normalized cell index of (a) motility and (c) invasion analyzed by xCELLigence RTCA system. Fold change graphs and brightfield images of (b) motile and (d) invaded cells in trans-well cell culture inserts. (e) Brightfield microscopy images of 0-, 24- and 48-h scratch (wound-healing) assay of MOCK and HOTAIR OE clones. All graphs of experiments are presented as the mean ± SE of at least 3 independent experiments. * p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001