Fig. 6.

lncRNA HOTAIR suppresses c-Met induced cell scattering, enhances survival under FSS and promotes metastasis in zebrafish embryos. Spheroid formation, survival under fluidic shear stress and metastatic capacity of MOCK and HOTAIR OE cells. (a) Spheroid images were collected with stereo-microscopy and magnified equally with scale-bars to generate more clarified presentation of data. (b) Graphical presentation of alive cell numbers of MOCK and HOTAIR OE clones cultured under 0.5 dyn/cm² fluidic shear stress for 1 h. Embryonic zebrafish xenograft experiments with MOCK and HOTAIR OE SNU-449 cell clones presented as (c) percentage of metastasis in groups that were analyzed in total of three biological experiments. Statistical significance was analyzed by chi-square with Yates correction. All graphs of experiments are presented as the mean ± SE of at least 3 independent experiments. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001