Fig. 7.

lncRNA HOTAIR maintains hybrid E/M phenotype to promote metastasis. (a) Schematic presentation of morphological, molecular and biological biomarkers of epithelial, mesenchymal and hybrid E/M phenotypes. Confocal imaging of (b) Alexa-555 labeled phalloidin staining of F-actin filaments (red). Immunofluorescence imaging of (c) beta-Catenin protein (red) and its cellular localization. Confocal imaging and RT-qPCR analysis of (d) E-Cadherin (e) Vimentin and (f) N-Cadherin expressions. Alexa-594 conjugated secondary antibodies were used in all immunolabeling and confocal imaging experiments. DAPI (blue) was used as a nuclear marker in immunofluorescence and confocal images. All graphs of experiments are presented as the mean ± SEM of at least 3 independent experiments. * p ≤ 0.05