Table 2.
Pathological and DNA sample information for NGS analysis
| Case no. | Sample no. | Samples | Pathological diagnosis | Storage time | DNA yield (ng/μL) | QC score | Input DNA (ng) | Tumor fraction in FFPE (%) | Tumor cell count and tumor cell ratio in LBC | Insufficient VAF call | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tumor cells (T) | Non-tumor cells (N) | Tumor cell ratio (T/N+T)% | ||||||||||
| 1 | 1 | SR(Ut) | EC G1 | 1 m | 1.655 | 0.012 | 200 | 30 | ||||
| 2 | 2 | SR(Ut) | EC G1 | 2 m | 5.510 | 0.003 | 100 | 80 | ||||
| 3 | 3 | SR(Ut) | EC G2 | 1 m | 2.980 | 0.008 | 100 | 60 | ||||
| 4 | 4 | SR(Ut) | EC G1 | 1 m | 1.521 | 0.016 | 200 | 50 | ||||
| 5 | 5 | SR(Ut) | EC G1 | 3 m | 4.033 | 0.010 | 119 | 80 | ||||
| 6 | 6 | SR(Ut) | EC G1 | 3 m | 6.636 | 0.003 | 72 | 30 | ||||
| 7 | SR(Ut) | EC G2 | 3 m | 6.071 | 0.004 | 79 | 60 | |||||
| 7 | 8 | SR(Ut) | EC G1 + Sq | 1 m | 5.912 | 0.003 | 200 | 70 | ||||
| 8 | 9 | SR(Ut) | Mixed EC/SC | 2 m | 3.487 | 0.010 | 138 | 50 | ||||
| 10 | SR(Om) | Mixed EC/SC | 2 m | 4.897 | 0.007 | 98 | 30 | |||||
| 9 | 11 | Bx | SC | 3 m | 6.425 | 0.004 | 75 | 80 | ||||
| 12 | SR(Ut) | CCC | 3 m | 1.930 | 0.015 | 168 | 90 | |||||
| 13 | End LBC | AC | 3 m | 28.879 | 0.000 | 20 | 6661 | 10,059 | 39.8 | |||
| 10 | 14 | SR(Ut) | DC | 3 m | 3.337 | 0.007 | 144 | 70 | ||||
| 15 | SR(Ut) | EC G1 | 3 m | 5.743 | 0.004 | 84 | 70 | |||||
| 11 | 16 | SR(Ut) | AC | 1 m | 2.327 | 0.011 | 168 | 60 | ||||
| 17 | SR(Ut) | DC | 1 m | 0.939 | 0.016 | 168 | 50 | |||||
| 18 | SR(Ov) | EC G3 | 1 m | 1.778 | 0.012 | 168 | 40 | |||||
| 12 | 19 | Bx | AC | 1 m | 2.264 | 0.010 | 168 | 60 | ||||
| 20 | SR(Ut) | EC G2 | 1 m | 3.025 | 0.006 | 168 | 70 | |||||
| 21 | End LBC | Malig | 2 m | 21.861 | 0.002 | 22 | 19,588 | 4087 | 82.7 | |||
| 13 | 22 | SR(Ut) | EC G1 | 1 m | 2.338 | 0.010 | 168 | 50 | ||||
| 14 | 23 | Bx | EC G1 | 1 m | 5.664 | 0.001 | 85 | 70 | ||||
| 24 | End LBC | AGC | 2 m | 23.839 | −0.003 | 10 | 1450 | 136 | 91.4 | |||
| 15 | 25 | SR(Ut) | DC | 1 m | 6.249 | 0.008 | 77 | 90 | ||||
| 26 | SR(Ut) | AC | 1 m | 5.830 | 0.010 | 85 | 90 | |||||
| 16 | 27 | Bx | AC | 1 m | 5.029 | 0.010 | 95 | 80 | ||||
| 28 | SR(Ut) | Serous | 1 m | 0.872 | 0.017 | 168 | 40 | |||||
| 29 | End LBC | AC | 2 m | 14.257 | 0.005 | 31 | 22,261 | 3928 | 85.0 | |||
| 17 | 30 | SR(Ut) | AH | 1 m | 3.443 | 0.007 | 140 | 70 | ||||
| 31 | SR(Ut) | EC G1 | 1 m | 4.137 | 0.006 | 116 | 60 | |||||
| 18 | 32 | Bx | EC G1 | 1 m | 3.794 | 0.009 | 127 | 90 | ||||
| 33 | SR(Ut) | EC G1 | 1 m | 3.025 | 0.012 | 168 | 80 | |||||
| 34 | End LBC | EC | 2 m | 3.392 | 0.008 | 11 | 8043 | 20,911 | 27.8 | * | ||
| 19 | 35 | SR(Ut) | EC G1 + Sq | 2 m | 4.585 | 0.004 | 105 | 30 | ||||
| 36 | End LBC | EC | 4 m | 16.984 | 0.000 | 26 | 7739 | 9261 | 45.5 | |||
| 20 | 37 | Bx | EC G2 | 3y | 0.492 | 0.022 | 168 | 60 | ||||
| 21 | 38 | Bx | EC G1 | 3 m | 2.612 | 0.012 | 168 | 90 | ||||
| 39 | SR(Ut) | EC G1 | 1 m | 1.321 | 0.025 | 154 | 90 | |||||
| 40 | End LBC | EC | 3 m | 16.114 | 0.005 | 36 | 16,855 | 10,739 | 61.1 | |||
| 22 | 41 | Bx | EC G3 | 2y | 1.024 | 0.022 | 168 | 40 | ||||
| 42 | End LBC | AGC | 1 m | 16.208 | 0.004 | 30 | 14,816 | 1019 | 93.6 | |||
| 23 | 43 | Bx | AC | 2 m | 3.479 | 0.010 | 138 | 90 | ||||
| 44 | SR(Ut) | EC G2 | 2w | 2.443 | 0.012 | 168 | 90 | |||||
| 45 | End LBC | EC | 2 m | 8.576 | 0.004 | 54 | 45,220 | 2537 | 94.7 | |||
| 24 | 46 | Bx | EC | 4 m | 2.443 | 0.009 | 168 | 80 | ||||
| 47 | SR(Ut) | EC G1 | 2 m | 5.058 | 0.005 | 95 | 80 | |||||
| 48 | SR(Ov) | EC G1 | 2 m | 1.694 | 0.011 | 168 | 70 | |||||
| 49 | End LBC | AC | 4 m | 24.088 | −0.001 | 31 | 21,930 | 6661 | 76.7 | |||
SRsurgical resection,BxEndometrial biopsy,LBCliquid-based cytology,EndEndometrial,UtUterus,OmOmentumOvOvary,ECEndometrioid carcinoma,SCSerous carcinoma,CCCClear cell carcinoma,ACAdenocarcinoma,SqSquamous differentiation,DCDedifferentiated carcinoma,MaligMalignant cell,AGCAtypical glandular cell,AHAtypical hyperplasia,w, week,mmonthy, year