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. 2020 Jul 11;20:303. doi: 10.1186/s12935-020-01395-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — MRTF-A has a positive role in the regulation of miR-155 expression in GC cells. MRTF-A over-expression upregulated miR-155 and MRTF-A RNA levels in SGC-7901 and MGC-803 cells (a, g). MRTF-A over-expression increased MRTF-A protein levels in SGC-7901 and MGC-803 cells (c, e). a, c, e, g MRTF-A encoding plasmids were transfected into gastric cancer cells; pcDNA3.1 was the vector control. Forty-eight hours after transfection, mRNA levels were determined by RT-qPCR and protein levels were determined by Western-blot. GAPDH was used as a loading control. MRTF-A depletion with siRNA reduced miR-155 and MRTF-A RNA levels in SGC-7901 and MGC-803 cells (b, h). MRTF-A depletion with siRNA suppressed MRTF-A protein expression in SGC-7901 and MGC-803 cells (d, f). b, d, f, h Cells were transfected with siMRTF-A duplexes. mRNA and protein levels were determined 48 h after transfection. i MRTF-A over-expression elevated miR-155 promoter activity in SGC-7901 gastric cancer cells. j siMRTF-A reduced miR-155 promoter activity in SGC-7901 gastric cancer cells. Data are shown as the mean ± S.E.M, based on three to six independent experiments. **, p < 0.01