Table 2.

Relative abundances of ruminal bacterial phyla affected by Saccharomyces cerevisiae fermentation products (SCFP) and a subacute ruminal acidosis (SARA) challenge as determined by 16S rRNA gene sequencing

Phylum	No SCFP		SCFP		SEM1	P-value
	Control	SARA	Control	SARA		SARA	SCFP	SARA* SCFP
Above 0.1% community
Bacteroidetes	46.84a	20.78b	40.57	32.49	4.72	< 0.01	0.44	0.02
Firmicutes	35.08	53.86	43.44	50.25	5.06	0.04	0.69	0.3
Proteobacteria	1.12	13.88	0.78	5.5	2.74	0.02	0.39	0.46
Spirochaetes	1.41x	0.11y	1.79x	0.69y	0.22	< 0.01	0.06	0.68
Tenericutes	1.85x	0.45y	1.84x	1.06y	0.24	< 0.01	0.24	0.22
Cyanobacteria	1.05a	0.41b	0.60a	0.44b	0.15	0.02	0.23	0.22
SR1	0.75	0.43	0.53	0.22	0.2	0.14	0.32	0.18
TM7	0.41	0.28	0.31	0.23	0.08	0.22	0.36	0.99
Between 0.01 and 0.1% of community
Verrucomicrobia	0.23a	0.02b	0.36a	0.04b	0.05	< 0.01	0.17	0.74
Actinobacteria	0.052	0.072	0.047	0.032	0.029	0.94	0.51	0.6
Chloroflexi	0.005	0.016	0.049a	0.009b	0.01	0.25	0.13	0.05
Elusimicrobia	0.033a	0.002b	0.042a	0.005b	0.013	0.02	0.8	0.87
Fibrobacteres	0.115	0.047	0.088	0.082	0.028	0.16	0.89	0.24
Lentisphaerae	0.095	0	0.042	0.011	0.021	0.16	0.89	0.24
Planctomycetes	0.021	0.016	0.046	0.008	0.013	0.13	0.54	0.26
Synergistetes	0.016	0	0.025	0.009	0.009	0.16	0.39	0.93

¹SEM standard error of mean

^{a, b}LSmeans with different letters within SCFP treatment (No SCFP or SCFP) differ (P < 0.05)

^{x, y}LSmeans with different letters within SCFP treatment (No SCFP or SCFP) tend to differ (P < 0.10)