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. 2020 Jul 7;14:2645–2655. doi: 10.2147/DDDT.S255823

Figure 1.

Figure 1

Baicalin reversed IL-1β-induced viability inhibition and pro-apoptosis of primary human OA chondrocytes. Primary chondrocytes were cultured for 24 h in a 96-well-plate and then treated with 10 ng/mL IL-1β alone for 12 h, or pretreated with 20 μM baicalin or 100 nM rapamycin for 4 h followed by co-treatment with 10 ng/mL IL-1β and 20 μM baicalin or 100 nM rapamycin for 12 h. (A) Cell viability was measured by CCK-8 assay. (B) Cell apoptosis was detected by flow cytometry assay. (C) The expressions of apoptosis-related proteins were determined by qRT-PCR (C) and Western blot (D) analysis. **p < 0.01 vs Control group; ##p<0.01 vs IL-β alone treatment; &p<0.05, &&p<0.01 vs IL-β+baicalin+NC in group.