Table 1.
Brief Conclusions of This Review
| Sources | Methods | The Most Effective Method(s) | The Easiest Method(s) | Reference |
|---|---|---|---|---|
| Cord blood | 1. Ficoll+Centrifuge 2. Hydroxyethyl Starch+Centrifuge 3. Dextrane+Centrifuge 4. Immunoselection 5. Osmotic Selection Method |
1, 4 | 4 | 17,19,21 |
| Amniotic fluid | 1. Filtering+Centrifuge 2. Immunoselection |
2 | 1 | 29,31 |
| Amniotic membrane | 1. Mincing+Flushing 2. Explant 3. Collagenase (type Ⅱ)+DNase 4. Trypsin+Collagenase (type I or IV) or/and DNase |
3 | 1 | 32,35,37 |
| Chorionic membrane | 1. Trypsin+Collagenase (type I) 2. Dispase+Collagenase (type IV) |
1, 2 | 1, 2 | 10,34,39,40 |
| Decidua | 1. Mincing+Flushing 2. Explant 3. Trypsin+Collagenase (type IV) 4. Collagenase (type I)+DNase 5.Trypsin+DNAse+Collagenase (type 1) 6. Collagenase (type 1)+DNase+Dispase |
5 | 1 | 32,42,44 |
| Chorionic villi | 1. Explant 2. Trypsin-EDTA 3. Trypsin+Explant 4. Collagenase (type I) |
3 | 1 | 45,47,49,50 |
| Wharton jelly | 1. Stirring in PBS 2. Explant 3. Trypsin+Collagenase 4. Collagenase (type B)+Hyaluronidase+Trypsin 5. Collagenase Type I+Collagenase Type IV+Hyaluronidase 6. Enzymatic+Explant |
5 | 1 | 4,52,56,59 |
| Umbilical cord vein | 1. Explant 2. Collagenase (type I or IV) |
1 | 2 | 60,63 |
| Umbilical cord arteries | 1. Explant 2. Collagenase |
1 | 2 | 17,60 |
| Umbilical cord lining cells | 1. Explant | — | — | 15 |
| Whole umbilical cord | 1. Explant 2. Collagenase |
1 | 2 | 60 |