Table 1. Primers used to reverse transcriptional quantitative PCR.
| Gene | Forward primer from 5' to 3' | Reverse primer from 5' to 3' |
|---|---|---|
| PRRX1A | ACAGCGTCTCCGTACAGCGC | AGTCTCAGGTTGGCAATGCT |
| PRRX1B | CATCGTACCTCGTCCTGCTC | GCCCCTCGTGTAAACAACAT |
| β-actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT |
| CD24 | CTCCTACCCACGCAGATTTATTC | AGAGTGAGACCACGAAGAGAC |
| CD44 | CTGCCGCTTTGCAGGTGTA | CATTGTGGGCAAGGTGCTATT |
| ALDH1 | GCACGCCAGACTTACCTGTC | CCTCCTCAGTTGCAGGATTAAAG |
| OCT4 | CTGGGTTGATCCTCGGACCT | CCATCGGAGTTGCTCTCCA |
| NANOG | TTTGTGGGCCTGAAGAAAACT | AGGGCTGTCCTGAATAAGCAG |
| SOX2 | GCCGAGTGGAAACTTTTGTCG | GGCAGCGTGTACTTATCCTTCT |
| TGF-β1 | CTGACGGCCACGAACTTCC | GCACTGACATTTGTCCCTTGA |
| TGF-β2 | CTGGATGCAGCCTATTGCTT | TGGTGAGCGGCTCTAAATCT |
| TGF-β3 | AAGTGGGTCCATGAACCTAA | GCTACATTTACAAGACTTCAC |
| E-cadherin | CGAGAGCTACACGTTCACGG | GGGTGTCGAGGGAAAAATAGG |
| N-cadherin | TCAGGCGTCTGTAGAGGCTT | ATGCACATCCTTCGATAAGACTG |
| Vimentin | GAGAACTTTGCCGTTGAAGC | TCCAGCAGCTTCCTGTAGGT |
PCR, polymerase chain reaction.