Table 3.
Ex vivo gene delivery for bone regeneration.
Vector | Cell | Gene | Species | Model | Reference |
---|---|---|---|---|---|
Adenovirus | Bone marrow-derived MSCs | BMP2 | Mouse | Radius bone defect | [56] |
Electroporation | Bone marrow-derived MSCs | BMP2 or BMP9 | Mouse | Ectopic bone | [57] |
Electroporation | Bone marrow and adipose-derived MSCs | BMP2 or BMP6 | Mouse | Ectopic bone | [18] |
Electroporation | Bone marrow-derived MSCs | BMP6 | Pig | Vertebral bone defect | [58] |
Electroporation | Adipose-derived MSCs | BMP6 | Rat | Vertebral bone defect | [59] |
Lentivirus | Umbilical cord blood -derived MSCs | BMP2 | Mouse | Ectopic bone | [60] |
Lentivirus | Bone marrow-derived MSCs (freshly - isolated or sub-cultured) | BMP2 | Rat | Femoral bone defect | [65] |
Adenovirus | Gingival fibroblasts | BMP2 | Rat | Calvaria bone defect | [61] |
Adenovirus | Periosteal cells | BMP2 | Rabbit | Mandible bone defect | [62] |
Baculovirus | Adipose-derived MSCs | BMP-2 and miR-148b | Mouse | Calvaria bone defect | [73] |
Polyethylenimine (PEI) | Bone marrow-derived MSCs | miRNA-20a and siRNA against noggin | Rat | Calvaria bone defect | [78] |
Baculovirus | Adipose-derived MSCs | BMP2 and CRISPR interference against noggin | Rat | Calvaria bone defect | [80] |