3D confocal images were taken at a magnification of 40x. The ivory color represents bone. The bone, LCN, and osteocyte membranes are semi-transparent in this image. The nuclei were labeled with DAPI, shown in blue, and X-Gal precipitate, which indicates activation of the osteocytes) is depicted in yellow. The nuclei and precipitate are opaque in this image, and typically appear tinted by the pink (LCN) and green (osteocyte) colors that surround them.