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Apparent Kinetic parameters for NpsA.
| Substrate | KM (μM) | kcat (s−1) | kcat/KM (M−1·s−1) |
|---|---|---|---|
| 3-Hydroxyanthranilic acid | (6.3 ± 0.2) × 101 | 0.78 ± 0.01 | (1.8 ± 0.1) × 104 |
| 3-Hydroxybenzoic acid | (4.0 ± 0.9) × 103 | 0.35 ± 0.07 | (8.8 ± 2.6) × 101 |
| Anthranilic acid | (8.5 ± 0.7) × 102 | 0.29 ± 0.01 | (3.4 ± 0.3) × 102 |
| ATP | (1.5 ± 0.1) × 101 | 0.82 ± 0.01 | (5.6 ± 0.4) × 104 |
| holo-ThdA | 3.4 ± 0.2, (Ki = 15 ± 2) | 1.08 ± 0.02 | (3.2 ± 0.2) × 105 |
