Fig.5. Genes in the 16-gene ACS signature that mediate protective immune responses in the mouse model.

Control or gene deficient mice were infected with Mtb HN878 (100 CFU) by aerosol route. On 30, 60 and 100 days post-infection (dpi), lungs were harvested to assess bacterial burden by plating, or inflammatory cell infiltration by flow cytometry, or histologically by H&E staining in (A) Statt^−/− mice (B) Tap1^−/− mice, (C) GbpChr3^−/− mice (D) Trafdt^−/− mice and (E) Sept4^−/− mice and relevant controls are shown. The data points represent the means (±SEM) of one out of two separate experiments. Significance for bacterial burden analysis for C57BL/6 and gene deficient mice was determined using unpaired two-tailed Student’s t-test at different dpi.