Table 1.
PCR System | Target Gene | Name of Primer | Sequence | Fragment Length | Tm °C | Source |
---|---|---|---|---|---|---|
Extraction control | Phage T4 | T4F | CCATCCATAGAGAAAATATCAGAACGA | - | 60 | [22] |
T4R | TAAATAATTCCTCTTTTCCCAGCG | |||||
T4probe | VIC-AACCAGTAATTTCATCTGCTTCTGATGTGAGGC | |||||
Adenoviruses | Hexon | AQ1 | GCCACGGTGGGGTTTCTAAACTT | - | 60 | [23] |
AQ2 | GCCCCAGTGGTCTTACATGCACAT C | |||||
AP | 6FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA | |||||
DNA polymerase | Fw Outer | TGATGCGYTTCTTACCTYTGGTYTCCATGAG | ≈1400 bps | 58 | [13] | |
Rw Outer | AGTTYTACATGCTGGGCTCTTACCG | |||||
Fw inere | GTGACAAAGAGGCTGTCCGTGTCCCCGTA | ≈250 bps | 55 | |||
Rw inere | TCACGTGGCCTACACTTACAAGCCAATCAC |
Tm: annealing temperature.