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. 2020 Jun 18;12(6):657. doi: 10.3390/v12060657

Table 1.

PCR systems used in the present study.

PCR System Target Gene Name of Primer Sequence Fragment Length Tm °C Source
Extraction control Phage T4 T4F CCATCCATAGAGAAAATATCAGAACGA - 60 [22]
T4R TAAATAATTCCTCTTTTCCCAGCG
T4probe VIC-AACCAGTAATTTCATCTGCTTCTGATGTGAGGC
Adenoviruses Hexon AQ1 GCCACGGTGGGGTTTCTAAACTT - 60 [23]
AQ2 GCCCCAGTGGTCTTACATGCACAT C
AP 6FAM-TGCACCAGACCCGGGCTCAGGTACTCCGA
DNA polymerase Fw Outer TGATGCGYTTCTTACCTYTGGTYTCCATGAG ≈1400 bps 58 [13]
Rw Outer AGTTYTACATGCTGGGCTCTTACCG
Fw inere GTGACAAAGAGGCTGTCCGTGTCCCCGTA ≈250 bps 55
Rw inere TCACGTGGCCTACACTTACAAGCCAATCAC

Tm: annealing temperature.