. 2020 Jun 23;12(6):677. doi: 10.3390/v12060677

Table 1.

Primer sets and primer efficiency for reverse transcriptase real time PCR (RT-qPCR).

Target Gene	Primer Sequence Forward and Reverse (5′–3′)	Efficiency (%)
RPL13A (reference gene)	TGGGCCGGAAGGTTGTAGTCGT	99
RPL13A (reference gene)	TTGCGGAGGAAGGCCAGGTAATTCA	99
IFN-λ1	TCCCTACTTCCAAACCCACC	95
IFN-λ1	GTTCTTCCAGGAGAGCGACT	95
IFN-λ2L	CGCCTCTTCCCTAGAAACCGGGACC	96
IFN-λ2L	CTCCAGGACCTTCAGTGTCAAGGCC	96
IRF7	GCAAGGTCTACTGGGAGGTG	97
IRF7	GTGCTGAAGTCGAAGATGGGG	97
CPV2 E6	ATATTTATGAAACCGTTAGCC	99
CPV2 E6	CGCAGCTGTCACAAGTGTTCC	99
CPV2 E7	ACAGAGAGAACCTGGGCGATA	100
CPV2 E7	ATAATGCCAAGCCCGTCTAA	100

RPL13A, ribosomal protein L13a; IFN, interferon; IRF, interferon regulatory factor; CPV2, canine papillomavirus 2.